Journal: Reproductive biology and endocrinology : RB&E

Article Title: Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

doi: 10.1186/1477-7827-2-29

Figure Lengend Snippet: Detection of low iNOS mRNA levels in fetal membranes (FM) by nested RT-PCR, in serum-free cultures. Lane 1 fetal membranes immediately after collection, lane 2 control (cultured membranes without PAF), lane 3 10 nM PAF, lane 4 positive control (CHOiNOS cells), lane 5 PCR blank (no template), lane 6 empty, lanes M ΦX174 DNA/Hae III marker. The expected size of the product was 262 base pairs. The faint band below the main band at 262 base pairs was present in all gels and appeared to be an artefact of nested amplification. This is one example of four identical results with four different samples of fetal membranes.

Article Snippet: Molecular weight markers for DNA, ΦX174 DNA/Hae III Marker (Promega, Southampton, UK), were run on the gels for reference.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Positive Control, Marker, Amplification

Journal: Reproductive biology and endocrinology : RB&E

Article Title: Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

doi: 10.1186/1477-7827-2-29

Figure Lengend Snippet: Induction of iNOS mRNA expression in human fetal membranes (FM) by culturing in the presence of serum for 24 hours. Lane 1 fetal membranes immediately after collection, lane 2 membranes cultured with serum, lane 3 membranes cultured with serum and 1 μM PAF, lane 4 positive control for iNOS, lane 5 PCR blank, M DNA markers. The expected size of the product was 310 base pairs. One example is shown of three identical results with three different samples of fetal membranes. All of the fetal membrane samples were initially negative for iNOS mRNA, as detected by the standard RT-PCR method.

Article Snippet: Molecular weight markers for DNA, ΦX174 DNA/Hae III Marker (Promega, Southampton, UK), were run on the gels for reference.

Techniques: Expressing, Cell Culture, Positive Control, Membrane, Reverse Transcription Polymerase Chain Reaction

Journal: Reproductive biology and endocrinology : RB&E

Article Title: Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

doi: 10.1186/1477-7827-2-29

Figure Lengend Snippet: Induction of iNOS mRNA expression in human fetal membranes (FM), cultured without serum, following treatment with LPS (1 μg/ml) alone or in combination with sodium butyrate (SB) (2 mM). Lane 1 FM control; lane 2 FM + LPS; lane 3 FM LPS + SB; lane 4 CHOiNOS positive control; lane 5 iNOS short standard; lane 6 PCR blank (no template); lane M ΦX174 DNA/Hae III Marker (Promega). Lanes 2–4 show characteristic iNOS bands at 310 base pairs (bp). One example is shown of four similar results with four different samples of fetal membranes

Article Snippet: Molecular weight markers for DNA, ΦX174 DNA/Hae III Marker (Promega, Southampton, UK), were run on the gels for reference.

Techniques: Expressing, Cell Culture, Control, Positive Control, Marker

Journal: Reproductive biology and endocrinology : RB&E

Article Title: Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

doi: 10.1186/1477-7827-2-29

Figure Lengend Snippet: No induction of iNOS mRNA expression in human fetal membranes (FM), cultured without serum, following treatment with PAF (10 nM). Lane 1 fetal membranes immediately after collection, lane 2 control, lane 3 = 10 nM PAF, lane 4 positive control, lanes M ΦX174 DNA/Hae III marker. This is one example of four identical results with four different samples of fetal membranes. The fetal membrane samples were initially negative for iNOS mRNA, as detected by the standard RT-PCR method.

Article Snippet: Molecular weight markers for DNA, ΦX174 DNA/Hae III Marker (Promega, Southampton, UK), were run on the gels for reference.

Techniques: Expressing, Cell Culture, Control, Positive Control, Marker, Membrane, Reverse Transcription Polymerase Chain Reaction